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Rye Berries are choice substrate for cubensis strains. Use a pot and measure out the grains 1 cup per quart mason jar. Rinse them in hot water several times until the water is clear, than fill the pot with hot water 2 inches above the rye. Then add 1/4 cup of gypsum per 5 cups of grain. Stir and let them sit for 12 - 18 hour to hydrate them. Than after soaking period bring them to a boil stirring a couple of times. Boil for 10 minutes than remove from pot and strain them. Give the strainer a shake to toss the grain so steam rises. The steam will cause the outside of the grains to become dry while leaving the inside moist. To achieve the proper moisture content you will get a napkin and put a little bit on it and wait 15 seconds after 15 seconds remove the grains, if the napkin is wet than give the grains a toss again until the napkin comes out dry. Once moisture content is correct fill the mason jars 3/4 of the way and put lid on. Pressure sterilize them for 90 minutes at 15 psi than leave them in the pressure cooker to cool for 18 -24 hours. Once cooled you can inoculate them or do grain transfers. If doing grain to grain transfers make sure to shake the jars to disperse the colonized grains. If inoculating with spore do not shake the jars. Put the jars to colonize until 100% complete.
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Making hommade substrate for mycology use
-1 cup measuring
-Vermiculite sold at Home Depot, Lowes, Walmart and Target
-Brown rice flour sold at Walmart and major grocery stores
-Foil 3"x 3"
-Wide Mouth mason w/lids jars any size as long as it is not regular style jars
-Large soup pot or Pressure cooker
-Steamer for bottom of pot
-Small nail or 1/8" drill bit
-Hammer if using no drill bit
-Distilled or Spring Water
First use a 1/8th drill bit or nail to poke or drill 4 holes on lid In this video I used 5 holes do not do that because it might dry out the water content in jars which stalls the colonizing of the mycelium. Boil jars and lids with band in water for 20 minutes than take out and set them upside down on a paper towel. Wipe off excess moisture from bands and lids.
Prepare your substrate by putting 2 cups of vermiculite, 1 cup brown rice flour, and 1 cup distilled or spring water (2 parts Vermiculite,1 part brown rice flour, 1 part water. You can make as much as you want as long as you follow the
2x1x1 part mixture. Mix Vermiculite and flour together first until well mixed with fork than slowly add water until consistancy is like damp sand. Fill jars with spoon very lightly without compacting substrate should be loose and airey, leave a 1/2" space from top of jar. Wipe down rim of jar with paper towel so no moisture is left, if you fail to do this the risk of contamination is can happen. Put a dry layer of only vermiculite on top of the damp substrate until it is flush with rim of jar. Wipe rim again to clean any dust off. Put lids on jars tight and cover them with foil. The purpose of the foil is to prevent water from dripping through the holes on the lid and ruining the moisture content. Too much moisture can cause mycelium to not grow. Too little can make substrate too dry which will cause no growth
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