Problems of the RNA World - Origin Of Life - Dr. Fazale Rana

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Three subsets of sequence complexity and their relevance to biopolymeric information - David L Abel and Jac...
Three subsets of sequence complexity and their relevance to biopolymeric information - David L Abel and Jack T Trevors: Excerpt: Genetic algorithms instruct sophisticated biological organization. Three qualitative kinds of sequence complexity exist: random (RSC), ordered (OSC), and functional (FSC). FSC alone provides algorithmic instruction...No empirical evidence exists of either RSC of OSC ever having produced a single instance of sophisticated biological organization...It is only in researching the pre-RNA world that the problem of single-stranded metabolically functional sequencing of ribonucleotides (or their analogs) becomes acute. *******www.biomedcentral****/content/pdf/1742-4682-2-29.pdf Doug Axe humorously dismantled one of the latest ploys by materialists to oversell their meager evidence for a "self-replicating RNA molecule" in this following article: Biologic Institute Announces First Self-Replicating Motor Vehicle - Doug Axe - Excerpt: "So, advertising this as “self-replication” is a bit like advertising something as “free” when the actual deal is 1 free for every 1,600 purchased. It’s even worse, though, because you need lots of the pre-made precursors in cozy proximity to a finished RNA in order to kick the process off. That makes the real deal more like n free for every 1,600 n purchased, with the caveats that n must be a very large number and that full payment must be made in advance." *******biologicinstitute****/2009/04/01/biologic-institute-announces-first-self-replicating-motor-vehicle/ Stephen Meyer points out that intelligence design was clearly required for even this meager result: Biological Information: The Puzzle of Life that Darwinism Hasn’t Solved - Stephen C. Meyer Thus, as my book Signature in the Cell shows, Joyce’s experiments not only demonstrate that self-replication itself depends upon information-rich molecules, but they also confirm that intelligent design is the only known means by which information arises. *******www.evolutionnews****//2009/06/biological_information_the_puz.html Stephen Meyer Responds to Fletcher in Times Literary Supplement - Jan. 2010 Excerpt: everything we know about RNA catalysts, including those with partial self-copying capacity, shows that the function of these molecules depends upon the precise arrangement of their information-carrying constituents (i.e., their nucleotide bases). Functional RNA catalysts arise only once RNA bases are specifically-arranged into information-rich sequences—that is, function arises after, not before, the information problem has been solved. *******www.evolutionnews****/2010/01/stephen_meyer_responds_to_flet.html Materialists have not even created all 4 "letters" of RNA by natural means: Response to Darrel Falk’s Review of Signature in the Cell - Stephen Meyer - Jan. 2010 Excerpt: Sutherland’s work only partially addresses the first and least severe of these difficulties: the problem of generating the constituent building blocks or monomers in plausible pre-biotic conditions. It does not address the more severe problem of explaining how the bases in nucleic acids (either DNA or RNA) acquired their specific information-rich arrangements. *******www.signatureinthecell****/responses/response-to-darrel-falk.php Stirring the Soup - May 2009 "essentially, the scientists have succeeded in creating a couple of letters of the biological alphabet (in a "thermodynamically uphill" environment). What they need to do now is create the remaining letters, and then show how these letters were able to attach themselves together to form long chains of RNA, and arrange themselves in a specific order to encode information for creating specific proteins, and instructions to assemble the proteins into cells, tissues, organs, systems, and finally, complete phenotypes." Uncommon Descent - C Bass: *******www.uncommondescent****/intelligent-design/stirring-the-soup/#comments Scientists Say Intelligent Designer Needed for Origin of Life Chemistry Excerpt: Organic chemist Dr. Charles Garner recently noted in private correspondence that "while this work helps one imagine how RNA might form, it does nothing to address the information content of RNA. So, yes, there was a lot of guidance by an intelligent chemist." Sutherland's research produced only 2 of the 4 RNA nucleobases, and Dr. Garner also explained why, as is often the case, "the basic chemistry itself also required the hand of an intelligent chemist." *******www.evolutionnews****/2009/07/scientists_say_intelligent_des.html#more Meyer Responds to Stephen Fletcher - Stephen Meyer - March 2010 Excerpt: Nevertheless, this work does nothing to address the much more acute problem of explaining how the nucleotide bases in DNA or RNA acquired their specific information-rich arrangements, which is the central topic of my book (Signature In The Cell). In effect, the Powner (Sutherland) study helps explain the origin of the “letters” in the genetic text, but not their specific arrangement into functional “words” or “sentences.” *******www.evolutionnews****/2010/03/meyer_responds_to_stephen_flet.html Origin of Life: Claiming Something for Almost Nothing Excerpt: Yarus admitted, “the tiny replicator has not been found, and that its existence will be decided by experiments not yet done, perhaps not yet imagined.” But does this (laboratory) work support a naturalistic origin of life? A key question is whether a (self-replicating) molecule could form under plausible prebiotic conditions. Here’s how the paper described their work in the lab to get this (precursor) molecule: RNA was synthesized by Dharmacon. GUGGC = 5’-GUGGC-30 ; GCCU – 5’P-GCCU-3’ ; 5’OH-GCCU = 5’-GCCU-3’ ; GCCU20dU = 5’-GCC-2’-dU; GCC = 5’-GCC-3’ ; dGdCdCrU = 5’-dGdCdCU-3’ . RNA GCC3’dU was prepared by first synthesizing 5’-O-(4,4’- Dimethoxytrityl)3’-deoxyuridine as follows: 3’-deoxyuridine (MP Biomedicals; 991 mg, 0.434 mmol) was dissolved in 5 mL anhydrous pyridine and pyridine was then removed under vacuum while stirring. Solid was then redissolved in 2 mL pyridine. Dimethoxytrityl chloride (170 mg, 0.499 mmol) was dissolved in 12 mL pyridine and slowly added to 3’-deoxyuridine solution. Solution was stirred at room temperature for 4 h. All solutions were sequestered from exposure to air throughout. Reaction was then quenched by addition of 5 mL methanol, and solvent was removed by rotary evaporation. Remaining solvent evaporated overnight in a vacuum chamber. Product was then dissolved in 1 mL acetonitrile and purified through a silica column (acetonitrile elution). Final product fractions (confirmed through TLC, 1.1 hexane:acetonitrile) were pooled and rotary evaporated. Yield was 71%. Dimethoxytrityl-protected 30dU was then sent to Dharmacon for immobilization of 30-dU on glass and synthesis of 5’-GCC-3’-dU. PheAMP, PheUMP, and MetAMP were synthesized by the method of Berg (25) with modifications and purification as described in ref. 6. Yield was as follows: PheAMP 85%, PheUMP 67%, and MetAMP 36%. Even more purification and isolation steps under controlled conditions, using multiple solvents at various temperatures, were needed to prevent cross-reactions. (then this understatement follows) It is doubtful such complex lab procedures have analogues in nature. *******www.creationsafaris****/crev201003.htm#20100302a Here are some more critiques of the "RNA World" scenario: Did Life Begin in an RNA World? *******www.creationsafaris****/crev0702.htm#abiogenesis62 Self Replication and Perpetual Motion - The Second Law's Take On The RNA World *******theory-of-evolution****/chap10/self-replicating-RNA-perpetual-motion-4.php The RNA World: A Critique - Gordon Mills and Dean Kenyon: *******www.arn****/docs/odesign/od171/ribo171.htm Intelligent Design - The Anthropic Hypothesis *******lettherebelight-77.blogspot****/
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